VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.
- Ideal choice for routine applications
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
Taq DNA polymerase concentration: 5 Units/μl
10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 1% Tween® 20
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3; KCl, 1% Triton™ X-100
EU = Units
Delivery information: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimising the need for optimisation of the Mg²⁺ concentration, or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.