Ni-NTA spin columns, HisPur™

Supplier: Thermo Scientific
Pierce HisPur™
PIER88224EA 233 EUR
PIER88224 PIER88225 PIER88226
Ni-NTA spin columns, HisPur™
Filters Centrifugal Filters
Pierce™ Ni-NTA spin columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

  • High capacity binding up to 60 mg of 6xHis-tagged protein per milliliter of resin
  • Purify proteins using native or denaturing conditions
  • Resin can be reused at least five times without reduction in performance
  • Purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates

The specially prepared support consists of beaded agarose derivatised with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilised metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
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