FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.
- Full and efficient protease digestion and recovery of peptides
- Glutaraldehyde-free silver stain that results in no lysine modification
- Protein cross linking or reduced peptide recoverys
- Protein can be detected in less than 90 min
- Sensitivity 0,3 ng
Delivery information: Supplied with SilverOUT™ to completely remove silver ions that inhibit protease digestion by binding at the active sites of proteases.