Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.
- High quality buffers
- 2.5% acetic acid elution buffer
- All buffer components listed and defined
- Convenient format
- Stock solutions can be diluted directly into the provided bottles for storage
- Eliminates time-consuming buffer preparation, which allows fast, reproducible protein enrichment
The buffers provided in the kit are optimized for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with Protein A HP SpinTrap, Protein G HP SpinTrap, and Ab SpinTrap columns. Kit includes 10x Binding/Washing Buffer [TBS]: 0.5 M Tris, 1.5 M NaCl, pH 7.5 Elution Buffer [Classic]: 2.5 % HAc, 10x Elution Buffer [Crosslink]: 1 M glycine-HCl, pH 2.9, 10x Crosslink Solution A: 2 M triethanolamine, pH 8.9, 10x Crosslink Solution B: 1 M ethanolamine, pH 8.9.