Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
- Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalisation
- Conjugates with wide choice of CF® dye colours, plus biotin, R-PE, APC, and other fluorescent dyes
- Biotium's CF® dyes have superior brightness, photostability, and are water-soluble
- Can be used for fluorescence microscopy, live cell imaging, and flow cytometry
Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35 to 36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labelled Annexin V.
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.