PCR products of up to 12 kb and up to 40 kb can be achieved with the high performance peqGOLD 'Mid Range' and 'Long Range' PCR Systems respectively, suitable for both genomic and episomal templates. Applicable in place of standard Taq DNA polymerase for PCR amplifications when longer fragments and larger amounts are desirable, including sequencing, mapping and λ-phage or cosmid studies.
NOTE: Some applications for which these products can be used are protected in certain countries by patent law. Because purchase does not include the acquisition of licenses for use within the scope of applications protected by patent law, the acquisition of relevant licenses may be necessary depending on the application and on the country of application.
Delivery information: Mid Range' or 'Long Range' PCR enzyme with concentration 5 units/ml; Dilution buffer: 20 mM Tris-HCl (pH 8,0), 100 mM KCl, 0,2 mM EDTA, 55% Glycerin, 0.5% Tween 20, 0,5% Nonidet P40 and 250 µg/ml Gelatine; 10X Reaction Buffer: 500 mM of Tris-HCl (pH 9,1), 140 mM (NH₄)₂SO₄ and 17,5 mM MgCl₂ (Mid Range kit) or 22,5 mM MgCl₂ (Long Range kit); 25 mM MgCl₂ for optimisation of the enzyme activity. Long Range kit also includes DMSO and 10% Tween 20.