Autophagy detection tests, CYTO-ID®

Supplier: Enzo Life Sciences
CYTO-ID®
ALEXENZ-51031-0050EA 376 EUR
ALEXENZ-51031-0050
Autophagy detection tests, CYTO-ID®
Assays Cellular Assays
CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

  • Protocol validated with known inhibitors and activators of autophagic activity
  • Rapidly quantifies autophagy in native heterogeneous cell populations
  • Eliminates need for transfection efficiency validation required with LC3-GFP transfection
  • Negligible staining of lysosomes reduces background seen with other dyes
  • High-throughput screening of activators and inhibitors of autophagy

Optimised through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes and autolysosomes (autophagolysosomes).

Ordering information: Contains CYTO-ID green detection reagent, Hoechst 33342 nuclear stain, autophagy inducer (rapamycin) chloroquine control and 10X assay buffer.
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