Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
- Reaction set up can be done at room temperature
- Reduces non-specific amplification and prevents primer degradation during set up
- Zero-time reactivation due to hot start technology
- Speed of the polymerase allows short extension times
- Robust reactions, minimal optimisation needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with Phusion® green buffer
Delivery information: Kit contains 2X Phusion green hot start II high-fidelity PCR master mix (provides 1,5 mM MgCl₂ in the final 1X concentration), nuclease-free water, and DMSO.
