The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.
- Intense light emission with low background: high signal ratio
- Low femtogram detection (10 to 15), allows detection of >10 fg protein on a dot blot and >1 pg on a Western blot
- Supplied with a novel blocking agent which allows a rapid blocking step and produces a clear background
- Suitable for nitrocellulose and PVDF membranes
A femtogram level sensitive immunodetection system allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1,2 dioxetane supersensitive detection reagent and a unique, proprietary combination of blocking agent (NAP-BLOCKER™) and washing buffers to ensure low background and high signal to background ratio. Optional horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat are also offered for a complete chemiluminescence detection system.
femtoLUCENT™ PLUS is offered as a complete immunodetection system with all of the critical reagents needed for Western blots or dot-blot analysis or as a reagent kit for those who prefer to customise their detection methods with the choice of their own secondary antibody conjugates.
Ordering information: femtoLUCENT™ PLUS reagent kit includes detection reagents for HRP or AP, NAP-BLOCKER™, a non animal protein blocking agent, femto-TBST™ washing buffer, and optional secondary antibodies.