Cell culture buffers, Corning®

Supplier: Corning
25-035-CIEA 73.57 EUR
Cell culture buffers, Corning®
Buffers Cell Culture Buffers
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO₂. Unlike the low pKa of bicarbonate, HEPES has a pKa of 7,66 making it a much stronger buffer in the pH 7,2 to 7,6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell. The recommended working concentration is 10 to 25 mM.
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