6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Product protocol provides an comparison of prestain, precast, and post-stain protocols for GelRed, and to find the recommended method for your application.
Prestain buffer with blue tracking dyes contains two blue electrophoresis tracking dyes that run at approximately 1,5 kb and 200 bp in a 1% agarose gel.
Prestain buffer with orange tracking dye contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.