Lyso-ID® red detection kit (GFP-Certified®)

Supplier: Enzo Life Sciences
ALEXENZ-51005-500EA 383 EUR
Lyso-ID® red detection kit (GFP-Certified®)
Assays Cellular Assays
For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

  • Novel acidic organelle-selective dye, suitable for live cell staining of lysosomes
  • Long wavelength red emission that is easily multiplexed with common fluorescent dyes (coumarin, FITC, Cyanine 3) and fluorescent proteins (BFP, CFP, GFP, YFP)
  • No photo conversion to a green emitting dye and no complicating metachromatic dual emission artifacts, facilitating analysis with GFP, FITC and other fluorescent probes
  • Highly resistant to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods

Lyso-ID® Red Lysosomal Detection Kit (GFP-Certified®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal. By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments.

The Lyso-ID® Red Lysosomal Detection Kit (GFP-Certified®)contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs).

The Lyso-ID® Red dye has been validated for utility in live cell imaging applications, showing an appropriate response to treatment with well characterized lysosomal perturbation agents. A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well.

Delivery information: Kit contains Lyso-ID® Red Detection Reagent, 50 µL, Hoechst 33342 Nuclear Staining, 50 µL, Chloroquine Control, 7.5 µmol and10X Assay Buffer, 15 mL.
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