NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: Enzo Life Sciences
ALEXENZ-51014-100EA 507 EUR
ALEXENZ-51014-100
NUCLEAR-ID® green cell cycle kit for flow cytometry
Assays Cellular Assays
For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

  • Easy staining protocol, simply add the dye and analyze by flow cytometry
  • Excited using common 488 nm laser source, generating a green ~530 nm emission
  • No permeabilization step or RNase treatment necessary
  • Cell cycle results independent of incubation time, temperature, dye and cell concentrations
  • Provides DNA content information in live or fixed cells
  • Monitor changes in cell cycle dynamics arising from drug treatment or other perturbations
  • Performance validated using a wide range of cell densities

This kit is suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels.

Nuclear-ID® Green Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. The kit is suitable for (1) determining the percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as to quantify cells in the sub-G1 phase prior to apoptosis, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. The kit contains sufficient reagents for approximately 100 flow cytometry assays. A control cell cycle perturbation agent, Nocodazole, is provided for monitoring changes in cell cycle dynamics. Potential applications for live-cell studies are in the determination of cellular DNA content and cell cycle distribution for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms.

Ordering information: Quantity: 100 assays Contents: Nuclear-ID® Green Cell Cycle Detection Reagent, 100 µl
Nocodazole Control, 10 µl
10X Assay Buffer, 15 ml
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