Cell migration BioGel assay and invasion assay, Collagen-I coatedSupplier: Enzo Life Sciences
ALEXENZKIT1100001EA 357 EURALEXENZKIT1100001
Cell migration BioGel assay and invasion assay, Collagen-I coated
Assays Cellular Assays
Reproducibly monitor real-time cell migration or invasion, with an automation-friendly design.
2- or 3D assay for investigating cell migration or cell invasion of adherent cell lines
The Cell migration BioGel assay and invasion assay is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration. Formatted for a 96-well plate, the assay uses a non-toxic biocompatible gel (BioGel) to form a cell-free zone on cell culture surfaces. After seeding cells into the 96-well plate, the BioGel dissolves permitting cells to migrate into the well centers.The Cell migration BioGel assay and invasion assay can also be used to monitor cell invasion. After cell seeding, a Collagen-I overlay can be added to create a 3-D extracellular matrix (ECM) for cell invasion. Cell invasion into the Detection Zone can be assessed by performing a Z-stack analysis.The Cell migration BioGel assay and invasion assay enables the use of automated liquid handling equipment for cell seeding and allows for unlimited access to wells from cell seeding through data readout. The Cell migration BioGel assay and invasion assay is designed to be used with any stain or labeling technique. Researchers can capture and quantify real-time cell migration data using inverted microscopes, High Content Screening (HCS) and High Content Imaging (HCI) instruments.The Cell migration BioGel assay and invasion assay system has been designed for use with adherent cell cultures. This assay has been successfully used with HT-1080 and MDA-MB-231 cell lines, and Human Umbilical Vein Endothelial Cells (HUVECs).
Ordering information: Contains cell migration BioGel 96-well plate, Collagen-I coated.