Cell migration assay, collagen-I coated

Supplier: Enzo Life Sciences
ALEXENZKIT1140001EA 546 EUR
ALEXENZKIT1140001
Cell migration assay, collagen-I coated
Assays Cellular Assays
Reproducibly monitor real-time cell migration, without the use of special instrumentation.

2D assay for investigating cell migration of adherent cell lines, on Collagen-I.

The Cell migration assay, Collagen-I coated is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration. Formatted for a 96-well plate, the assay utilizes Cell Seeding Stoppers made from a medical-grade silicone to restrict cell seeding to the outer annular regions of the wells. Removal of the stoppers reveals a 2mm diameter unseeded region in the center of each well, i.e., the detection zone, into which the seeded cells may then migrate. The Detection Mask is applied to the plate bottom and restricts visualization to the detection zones, allowing only cells that have migrated to be detected. The Cell migration assay is designed to be used with any stain or labeling technique. Readout can be performed by microscopy or use of a microplate reader.The Cell migration assay, Collagen-I coated system has been designed for use with adherent cell cultures. This assay has been successfully used with HT-1080, PC-3, A549, NCI H1650, MDA-MB-231, NMuMG, 3T3-Swiss albino, HCEC, HUVEC, and MCF10A cell lines.

Ordering information: Contains Collagen-I coated 96-well plate (black, clear bottom) with cell seeding stoppers, cell migration detection mask, cell migration stopper tool.
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