Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.
AMPIGENE® qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE® qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR.An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE® qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Hi-ROX, see Prod. No. ENZ-NUC109.
Ordering information: Contains 2x AMPIGENE® qPCR 1-Step Green Mix Hi-ROX (includes dNTPs and polymerases) 20x RTase (with RNase inhibitor).