Bio-16-UTP

Supplier: Enzo Life Sciences
ALEXENZ-42814EA 390 EUR
ALEXENZ-42814
Bio-16-UTP
Nucleic Acid Reagents Nucleotides
Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

  • Appearance: Clear, colorless liquid
  • Purity: ≥93% (HPLC)
  • Concentration: 10 mM
  • Extinction coefficient: 10700 M⁻¹ cm⁻¹(240 nm, pH 7,0)

The biotin-labelled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridisation, sequencing, and genome analysis.

The biotin-labelled hybridised probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble coloured precipitate.
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