Bio-11-CTP

Supplier: Enzo Life Sciences
ALEXENZ-42818EA 390 EUR
ALEXENZ-42818
Bio-11-CTP
Nucleic Acid Reagents Nucleotides
Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

  • Appearance: Clear, colourless liquid
  • Purity: ≥93% (HPLC)
  • Concentration: 10 mM
  • Extinction coefficient: 10700 M⁻¹ cm⁻¹ (240 nm, pH 7,0)

The biotin-labelled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridisation, sequencing, and genome analysis.

The biotin-labelled hybridised probes can be detected by a reporter molecule linked to streptavidin, avidin, or anti-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble coloured precipitate.
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