Lipopolysaccharides (from E.coli) serotype R515 (Re), TLRgrade™Supplier: Enzo Life Sciences
Rough (R)-form LPS isolated and purified from E. coli R515 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form.
Strong activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
Specification Test Results
|Formulation||Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water|
|Purity||Absence of detectable protein or DNA contaminants with agonistic TLR activity|