NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical
CAYM10007889-96EA 640 EUR
NF-κB (p65) transcription factor assay kit
Assays Cellular Assays
NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

  • A sensitive, non-radioactive method of detecting NF-κB (p65) from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format

Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50).

Ordering information: Nuclear extraction kit is available separately to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates.

Delivery information: Kit contains: Binding assay buffer, Reagent A, positive control, antibody binding buffer, primary antibody, buffer concentrate, polysorbabte 20, competitor dsDNA, HRP conjugate, strip plate, cover sheet, developing and stop solution.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR