The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.
- Efficient removal of contaminating DNA from RNA preparations
- Free from RNases and inhibitors
Reaction time: 15 to 20 minutes on column at 20 to 30 °C
Activity: 10000 Kunitz units/mg
One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0,001 per minute per ml at 25 °C, pH 5,0, with highly polymerized DNA as the substrate.
Delivery information: Kit contains RNase-free DNase I and DNase I digestion buffer.
Caution: Storage/stability: Store at −20 °C. The peqGOLD DNase I Digest Kit is stable for at least 12 months from the date of purchase when both DNase I and DNase Digestion Buffer are stored at −20 °C.