Fast HiFi DNA polymerase, 2 U/µl

Supplier: VWR Life Science
733-2883EA 1980 EUR
733-2883 733-2882 733-2881 733-2880
Fast HiFi DNA polymerase, 2 U/µl
Nucleic Acid Reagents End-point PCR Enzymes and Kits
Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

  • High fidelity: >60X Taq Fidelity
  • 3’→5’ proof reading exonuclease activity
  • Processes up to 18 kb from complex templates (e.g. human gDNA)
  • Good coverage on DNA templates with low to high GC content
  • Fast extension rate: 10 s/kb

Fast HiFi DNA Polymerase 2 U/µl exhibits both 5'→3' DNA polymerase activity and 3'→5' proof reading exonuclease activity enabling this polymerase to correct base pair mismatches. It is supported with 5X Fast HiFi Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.

For more convenient handling Fast HiFi DNA Polymerase 2 U/µl is also available as a 2X master mix.

Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates
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