A DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences.
- Probes for nucleic acid hybridisation
- Templates for in vitro translation
- Substrates for RNA processing studies
- Exon and intron mapping of genomic DNA
SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesises RNA 5' to 3' and incorporates ³⁵S, ³²P and ³³P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone. SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity.
Unit definition: One unit will catalyze the incorporation of 1 N mole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37 °C.;Reaction Conditions: 40 mM Tris-HCl (pH 8,0), 8 mM MgCl₂, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25 µl volume. Incubate 60 minutes at 37 °C.
Caution: For research use only. Not for use in diagnostic procedures.