Rapidly and easily produce high yields of RNA from a DNA template.
- Produces transcripts from a wide range of DNA template sizes, up to 9 kb
- RNA yield 10 to 30 times the yield produced by conventional methods
- 1 μg DNA template is 100 μg plus RNA transcript
- For use in RNAi, microinjection studies, RNA-binding protein studies, hybridisation studies, and microarrays
The RNAMaxx high yield transcription kit produces large quantities of RNA quickly and easily from a DNA template. Just 1 µg of template can yield 80 to 100 µg or more of RNA transcript in a short incubation. This is 10 to 30 times the yield produced by any conventional in vitro transcription reaction. The kit uses T7 RNA polymerase to produce high yields of RNA in just two hours.
RNAMaxx is ideal for the construction of non-isotopically labeled RNA probes. A variety of modified nucleotides, including amino allyl- and biotin-labeled nucleotides, are easily incorporated. The resulting labeled RNA transcripts can be used as probes for microarrays, Northern blot analysis, or in situ hybridisation.
Caution: For research use only. Not for use in diagnostic procedures.