Native barcoding expansionSupplier: Oxford Nanopore Technologies
- Multiplex samples to reduce price per sample
- PCR-free multiplexing — preserve and analyse additional information such as base modifications
- High sequencing yields
- Control over read length
- Suitable for upstream processes such as size selection or whole-genome amplification
Native Barcoding Expansion 13-24 provides 12 unique barcodes (different to those provided in the Native Barcoding Expansion 1-12) with sufficient reagents for six sequencing libraries.
Native Barcoding Expansion 96 provides 96 unique barcodes with sufficient reagents for 12 sequencing libraries.
The library preparation method is similar to the Ligation Sequencing Kit protocol; DNA ends are repaired and dA-tailed using the NEBNext End Repair/dA-tailing module, and then a unique dT-tailed barcode adapter is ligated on the dA-tailed template. Barcoded samples are then pooled together. Each barcode adapter also has a cohesive end and this is used as a hook to ligate to the supplied sequencing adapters.
Native Barcoding Expansion packs are compatible with upstream processes such as whole genome amplification (for applications where under 1 ng of sample is available) and size selection (for enrichment of specified fragment lengths, using the BluePippin, for example). When size selecting, we recommend increasing the amount of input used, as size selection can be a wasteful process.
Native Barcoding Expansion packs can be used together with: Ligation Sequencing Kit (76487-138); Direct cDNA Sequencing Kit (76487-162); Flow Cell Wash Kit (76487-116).
Sample multiplexing for Native Barcoding Expansion 1-12 and Native Barcoding Expansion 13-24 is ≤12, while Native Barcoding Expansion 96 is ≤96.