Cell lysis buffer is used to lyse cells under non-denaturing conditions.
- Ready to use for all types of human or animal cells
- Lyse cells under non-denaturing conditions
- Protease inhibitors may be recommended, not included in product composition
If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice.
For adherent cells add 400 µl of buffer per 10 cm flask once they have been washed in 1X PBS.
For suspended cells add 400 µl of buffer per million cells once they have been washed in 1X PBS and pelleted.
For tissue lysates add 20 µl of buffer per 1 mg of tissue followed by homogenisation and sonication.
Application note: The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.