Flongle is an adapter for MinION devices that enables direct, real-time DNA sequencing on smaller, single-use Flongle Flow Cells.
- The most rapid, accessible, and cost-efficient nanopore sequencing system for smaller or more frequently performed assays and experiments
- Each Flongle Flow can deliver up to 2,8 Gb of real-time sequencing data
The Flongle Adapter allows MinION devices to run smaller, single-use Flongle Flow Cells, generating up to 2,8 Gb of data per flow cell. Users can simply insert a Flongle Flow Cell into the Flongle Adapter, run their experiment on their MinION device and then discard the Flongle Flow Cell and replace it with another.
How flow cells work: The user adds their prepared library to the flow cell and starts their sequencing experiment. Data is accumulated over time as DNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.
As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~24 hours (under optimal conditions).
Flongle R9.4.1 Flow Cells have been tested with the following sequencing kits: Ligation Sequencing Kit (SQK-LSK110), Ligation Sequencing Kit (SQK-LSK109), Rapid Sequencing Kit (SQK-RAD004), Rapid Barcoding Kit (SQK-RBK004), Rapid Barcoding Kit 96 (SQK-RBK110.96), 16S Barcoding Kit 1-24 (SQK-16S024).
Flongle R9.4.1 Flow Cells have been tested with the following Native Barcoding Expansion packs:Native Barcoding Expansion 1-12 (EXP-NBD104), Native Barcoding Expansion 13-24 (EXP-NBD114).
Theoretical max output (TMO). Assumes system is run for 16 hours at 420 bases/second. Actual output varies according to library type, run conditions, etc. TMO noted may not be available for all applications or all chemistries.
Delivery information: The Flongle Adapter is stored and shipped at ambient temperature (15 to 25 °C).
Flow cells and kits are shipped together at 2 to 8 °C. The packaging is designed to protect the flow cells from freezing.
Flongle Flow Cells can be stored, unopened, at 2 to 8 °C for four weeks.
Packaging: Flongle flow cells are packed individually.
Caution: Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.