Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
- High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity
- Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection
- Compatible with Rainbow™ molecular weight markers and Amersham™ ECL™ DualVue™ Western blotting markers
- Optimised for use with Amersham™ Hybond™ P (PVDF) membranes and compatible with Amersham™ Hybond™-ECL™ (nitrocellulose) membranes
Delivery information: Includes Solution A (luminol solution) and Solution B (peroxide solution), sufficient for 1000 cm² or 3000 cm² membrane.