AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.
AccuStart™ Genotyping ToughMix® contains all required reaction components, except primers, probes, and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product. AccuStart™ Genotyping ToughMix® is compatible with both fast and conventional PCR cycling protocols.
Delivery information: Kits are available with or without ROX reference dye. All kits comprise of 2X reaction buffer containing optimised concentrations of MgCl?, dNTPs (dATP, dCTP, dGTP, dTTP), hot start DNA polymerase, AccuVue™ blue qPCR dye, and stabilisers.