Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of Taq DNA polymerase, and 6-fold lower than that of Pfu DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than Pfu DNA polymerase.
- Extremely accurate and fast amplification
- Increased processivity allows shorter reaction times (extension 15 to 30 s/kb)
- Fewer reaction failures and minimal optimisation
- Increased product yields with minimal enzyme amounts
Delivery information: Phusion® High-Fidelity DNA Polymerase is supplied with 5X Phusion® HF Buffer, 5X Phusion® GC Buffer, DMSO and 50 mM MgCl₂ solution. Both Phusion® HF Buffer and Phusion® GC Buffer contain 7,5 mM MgCl₂ in the provided 5X concentration.
